Research Interests
Dr.
Chackerian's laboratory is interested in vaccine development;
particularly the use of virus particles as platforms for antigen
display. It has long been recognized that highly dense repetitive
antigens such as virus particles induce strong immune responses.
However, more recent studies from Dr. Chackerian's laboratory has
demonstrated that antigens that are normally poorly immunogenic
can be made highly immunogenic by displaying them in a multivalent,
repetitive format on the surface of virus particles; essentially using
viruses as platforms for vaccines. Dr. Chackerian has shown that
this ability to enhance immunogenicity does not only apply to epitopes
derived from traditional targets, such as pathogens, but also to
self-antigens which are normally subject to the mechanisms of B cell
tolerance. Using virus-like particles derived from RNA
bacteriophage, he has been collaborating with Dr. David Peabody (also a
member of MGM) to develop and implement a system that allows vaccines
to be rapidly identified by affinity selection. He also has
funded projects to develop novel vaccines targeting Human
Papillomavirus and HIV.
Selected Publications
Link to PubMed
Hunter, Z., Tumban, E., Dziduszko, A.,
and B. Chackerian (2011). Aerosol
delivery of Virus-like particles to the genital tract induces local and
systemic antibody responses. Vaccine. May 5. [Epub ahead of print]
Chackerian, B., Caldeira, J. D., Peabody, J., and D. S. Peabody
(2011). Peptide Epitope Identification by Affinity Selection on
Bacteriophage MS2 Virus-like Particles. Journal of Molecular
Biology. Jun 3;409(2):225-37. Epub 2011 Apr 9.
Ashley, C. E., Carnes, E. C., Phillips, G. K., Padilla, D., Durfee, P.
N., Brown, P.A., Hanna, T.N., Liu, J., Phillips, B., Carter, M. B.,
Carroll, N. J., Jiang, X., Dunphy, D. R., Wilman, C.L., Petsev, D.N.,
Evans, D. G., Parikh, A. N., Chackerian, B., Wharton, W., Peabody,
D.S., and C. J. Brinker (2011). The Targeted Delivery of Multicomponent
Cargos to Cancer by Nanoporous Particle-Supported Lipid Bilayers.
Nature Materials, May; 10(5): 389-397. Epub 2011 Apr 17.
Caldeira, J.D., Medford, A., Kines, R.C., Lino, C.A., Schiller, J.T.,
Chackerian, B., and D.S. Peabody (2010). Immunogenic Display of
Diverse Peptides, Including a Broadly Cross-Type Neutralizing Human
Papillomavirus L2 epitope, on Virus-like Particles of the RNA
Bacteriophage PP7. Vaccine, 28 (27), 4384-4393. Epub 2010 Apr 29.
Li, Q.Y. Gordon, M. N., Chackerian, B., Alamed, J., Ugen, K. E., and D.
Morgan (2010). Virus-like Peptide Vaccines Against Aß N-terminal
or C-terminal Domains Reduce Amyloid Deposition in APP Transgenic Mice
without Addition of Adjuvant. Journal of Neuroimmune
Pharmacology, 5 (1), 133-42.
Hunter, Z., Smyth, H. D., Durfee, P., and B. Chackerian (2009).
Induction of mucosal and systemic antibody responses by aerosol
delivery and intramuscular immunization of Virus-like Particle based
vaccines targeting CCR5. Vaccine, 28 (2), 403-414. Epub 2009 Oct
20.
Peabody,
D. S., Manifold-Wheeler,
B.,
Medford, A., Jordan, S. K.
Caldeira, J., and B. Chackerian (2008). Immunogenic Display of Diverse
Peptides on Virus-Like Particles of RNA Phage MS2. Journal of Molecular
Biology, 380, 252-263.
Chackerian, B. Durfee, M. R., and J.
T. Schiller (2008). Virus-like Display of a Neo-self Antigen Reverses B
cell Anergy in a B cell Receptor Transgenic Mouse Model. Journal of
Immunology, 180 (9), 5816-5825. B. Chackerian (2007). Virus-like
Particles: Flexible Platforms for Vaccine Development. Expert
Reviews of Vaccines 6 (3), 381-390.
Chackerian, B., Rangel, M., Hunter,
Z., and D. S. Peabody (2006).
Virus and virus-like particle based immunogens for Alzheimer's disease
induce antibody responses against Amyloid-Beta without concomitant T
cell responses. Vaccine 24 (37-39), 6321-6331.
Chackerian, B., Briglio, L., Albert,
P. S., Lowy, D. R., and J. T.
Schiller (2004). Induction of autoantibodies to CCR5 in macaques
and subsequent effects upon challenge with an R5-tropic Simian/Human
Immunodeficiency Virus. Journal of Virology 78 (8), 4037-4047.
Li, Q., Cao, C., Chackerian, B.,
Schiller, J. T., Gordon, M., Ugen, K.
E., and D. Morgan (2004). Overcoming Antigen Masking of
Anti-Abeta Antibodies Reveals Breaking of B cell Tolerance in Abeta
Immunized Amyloid Precursor Protein Transgenic Mice. BMC
Neuroscience 5: 21 (8 June 2004).
Chackerian, B., Lenz, P., Lowy, D.
R., and J. T. Schiller (2002).
Determinants of autoantibody induction by conjugated papillomavirus
virus-like particles. Journal of Immunology 169 (11), 6120-6126.
Chackerian, B., Lowy, D. R., and J.
T. Schiller (2001).
Conjugation of a self-antigen to papillomavirus-like particles allows
for efficient induction of protective autoantibodies. Journal of
Clinical Investigation 108 (3), 415-423.
Chackerian, B., Lowy, D. R., and J.
T. Schiller (1999). Induction
of auto-antibodies to mouse CCR5 with recombinant papillomavirus
particles. Proceedings of the National Academy of Sciences USA 96
(5), 2373-2378.
