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Image 3 - series
1st Place: Live Cell Time Series
Yang Wu Pathology
U937 cells stably expressing GFP-tagged formyl peptide receptor (green). The addition of octadecyl Rhodamine B (appearance of red fluorescence) leads to the incorporation of octadecyl Rhod. B into both the plasma membrane and internalized vesicles. Subsequent addition of KI quenches red fluorescence everywhere except in internalized vesicles. Confocal fluorescence images merged with DIC image.
Acquired on BioRad Radiance Confocal/Nikon inverted microscope
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Image 4 -series
2nd Place: Live Cell Time Series
Diego Ramallo Pardo Center for High Tech Materials (CHTM)
Fluorescent microspheres moving slightly in fluid current indicate tethering to DNA. In contrast, microspheres stuck to the substrate do not move at all and free floating microspheres move through the field under the influence of an applied current.
Acquired on Nikon inverted microscope/Hamamatsu Orca digital camera |
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Image 1
1st Place: Spectral Image, Unmixed
DeAnna López Chemical & Nuclear Engineering
Staphylococcus aureus (green & red) immobilized in silica matrix (dark blue). Using the spectral detector allowed the separation of GFP expression of activated S. aureus from green autofluorescence of the silica matrix.
Acquired with spectral detector on Zeiss LSM510 Meta Confocal
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Image 2
1st Place: Brightfield
Naveed Saqib General Surgery
Acquired on Zeiss Axioskop microscope/Axiocam color digital camera |
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Image 5
1st Place: Widefield Fluorescence
Richard Smrt Neurosciences
Cluster of primary mouse neurons expressing Map2(red) also labeled with DAPI (blue).
Acquired with 20x objective on Zeiss Axioskop microscope/Axiocam digital camera |
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Image 6
2nd Place: Widefield Fluorescence
Leyma DeHaro Molecular Genetics and Microbiology
HEK293 cell nuclei labeled with DAPI (blue) and phosphorylated histone H2AX (red) following irradiation.
Acquired on Zeiss Axioskop microscope/Axiocam digital camera |
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Image 7
1st Place: Confocal Z-series, single channel (single-image projection)
Richard Smrt Neurosciences
GFP-expressing cells (green) from CA1 region of hippocampus (mouse brain section)
Acquired on Zeiss LSM510 Meta Confocal (63x objective) |
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Image 8
2nd Place: Confocal Z-series, single channel (single-image projection)
Brit Ventura Neurosciences
Tissue section of dentate gyrus of mouse hippocampus labeled in red with an antibody that recognizes TIMP-3 (tissue inhibitor of metalloproteinase 3)
Acquired on Zeiss LSM510 Confocal |
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Image 9
1st Place: Confocal Z-series, two channels (single-image projection)
Jessica Seidel Neurosciences
Tissue section of mouse hippocampus injected with lentivirus encoding CNTF. Shown are nuclei of all cells (DAPI, blue) and activated, enlarged astrocytes (GFAP labeled in red). Acquired on Zeiss LSM 510 Meta Confocal
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Image 10
2nd Place: Confocal Z-series, two channels (single-image projection)
Richard Smrt Neurosciences
Tissue section of mouse hippocampus in which a subset of CA1 pyramidal cells express an X-chromosome-linked GFP (green). All nuclei are labeled with DAPI (red). Acquired on Zeiss LSM 510 Meta Confocal using 40x objective
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Image 11
1st Place: Confocal Z-series, three channels (single-image projection)
Richard Smrt Neurosciences
Tissue section of mouse hippocampus in which a subset of CA1 pyramidal cells express an X-chromosome-linked GFP (green). Effects of the protein MeCP2 (red) on X chromosome inactivation are being studied. All nuclei are labeled with DAPI (blue). Acquired on Zeiss LSM 510 Meta Confocal using 40x objective
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Image 12
2nd Place: Confocal Z-series, three channels (single-image projection)
Brit Ventura Neurosciences
Sagittal section of rostral migratory stream in mouse brain showing migrating neuroblasts (FITC-green), vascular endothelial cells (Cy3-red) and nuclei of all cells (TOPRO-3 – blue). Acquired on Zeiss LSM510 Confocal
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Image 13
1st Place: Confocal Single Plane, single fluorescence channel
Matloob Husain Lovelace Respiratory Research Institute
Air-liquid interface cultures of differentiated NHBE cells showing healthy, elongated cilia (Alexa 594-red). Acquired on Zeiss LSM510 Meta Confocal
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Image 14
2nd Place: Confocal Single Plane, single fluorescence channel
Jacob Agola Pathology
BHK cells transfected with Rab 7. Red fluorescence (rhodamine) is associated with the membrane of late endosomes. Acquired on Zeiss LSM510 Confocal
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Image 15
1st Place: Confocal Single Plane, two fluorescence channels
Whitney Petrie Cell Biology and Physiology
Tissue section of mouse uterus. b-catenin in intercellular junctions is labeled green; nuclei are labeled blue. Acquired on Zeiss LSM510 Meta Confocal |
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Image 16
2nd Place-TIE: Confocal Single Plane, two fluorescence channels
Matloob Husain Lovelace Respiratory Research Institute
Air-liquid interface cultures of differentiated NHBE cells showing healthy, elongated cilia (Alexa 594-red) and Hoechst-labeled nuclei (blue). Acquired on Zeiss LSM510 Meta Confocal |
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Image 17
2nd Place-TIE: Confocal Single Plane, two fluorescence channels
Jessica Seidel Neurosciences
Tissue section of mouse hippocampus injected with lentivirus encoding CNTF. Shown are nuclei of all cells (DAPI, blue) and MAP2 in neuronal processes (green). Acquired on Zeiss LSM 510 Meta Confocal. |
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Image 18
1st Place: Confocal Single Plane, three fluorescence channels
Matloob Husain Lovelace Respiratory Research Institute
HeLa cells transfected with SARS coronavirus M protein. M Protein (Alexa488-green) and the Golgi marker bCOP1 (Alexa594-red) are colocalized (yellow) in transfected cells.. Nuclei are labeled with Hoeschst (blue). Acquired on Zeiss LSM510 Meta Confocal |
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Image 19
2nd Place: Confocal Single Plane, three fluorescence channels
Richard Smrt Neurosciences
40 mm tissue section of the dentate gyrus of mouse hippocampus in which a subset of CA1 pyramidal cells express an X-chromosome-linked GFP (green). Effects of the protein MeCP2 (red) on X chromosome inactivation are being studied. All nuclei are labeled with DAPI (blue). Acquired on Zeiss LSM 510 Meta Confocal using 40x objective |
