University of New Mexico School of Medicine
2325 Camino de Salud
Albuquerque, NM 87131
 
 
 
 

Immunofluorescence Protocols From UNM Research Labs

Wandinger-Ness Lab

Adherent Cells – Triton Permeabilization     word | pdf

Adherent Cells – Saponin Permeabilization (preserves intracellular membranes)     word | pdf

Shuttleworth Lab

Tissue Sections (12 – 60μm)     word | pdf

Tissue Sections (THICK 250μm)     word | pdf

Oliver-Wilson Lab

Adherent Cells     word | pdf

Additional Protocols

  1. Overview – Fixation, Permeabilization, Staining, and Mounting (with protocols)
    UBC BioImaging Facility
  2. Non-adherent cells – Fixation/Staining
    BD Biosciences
  3. Adherent Cells – Fixation/Staining
    King’s College, London
  4. Adherent Cells – Fixation/Staining (Click on Facilities and scroll to bottom of page.)
    Indiana University Center for Biological Microscopy
  5. Immunohistochemisty World - About one million protocols!
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