UNM College of Pharmacy
Dept. of Pharmaceutical Sciences
Albuquerque NM 87131-0001
2703 Frontier NE, suite 220
Department of Pharmaceutical
Office: Nursing/Pharmacy Building, room 184
Areas of Expertise
Microparticle preparation containing single/multiple drugs by spray drying (Buchi mini spray dryer) and double emulsion method.
Vaccine and drug formulation for inhaled therapy
Aerosol vaccination of dry powders to the lungs of animal models for Tuberculosis, Diphtheria and Hepatitis B and analyzing the immune response.
Aerosol drug delivery to the lungs of animal models and performing pharmacokinetic studies in plasma and lung tissues.
Cell-specific drug targeting
Inhalation delivery of microparticles to guinea pigs using nose-only inhalation apparatus (In- Tox)/ insufflator/ microsprayer (Penn Century).
Sampling techniques such as bronchoalveolar lavage, blood withdrawal from cardiac puncture, retro orbital puncture, inferior vena cava, saphenous vein, jugular vein cannulation.
Isolation of cells from tissues by density gradient centrifugation and magnetic bead separation.
Development and validation of extraction and analytical methods of drugs by HPLC/ LC-MS in formulation and bio-matrix (lung tissues, serum/ plasma, alveolar macrophages)
Experience with cell lines (J774 A.1, THP-1, NIH 3T3, CCRF) and with pathogenic (H37Rv) and non-pathogenic (H37Rv) strains of Mycobacterium tuberculosis.
Estimation of antibody and cytokine profile (Flow cytometry and ELISA).
Determination of aerosol characteristics using various cascade impactors.
Skills in using various
microscopic techniques (Confocal, SEM)
R. Sharma, A. B. Yadav, P. Muttil, H. Kajal and A. Misra Inhalable microparticles modify cytokine secretion by lung macrophages of infected mice Tuberculosis (2011) 91 (1), 107-110
P. Muttil, B. Pulliam, L. Garcia Contreras, J. Fallon, C. Wang, A.J. Hickey and D. A. Edwards Pulmonary Immunization of Guinea Pigs with Diphtheria CRM-197 antigen as Nanoparticle Aggregate dry-powders enhance Local and Systemic Immune Responses AAPS journal (2010) 12 (4), 699-707
P. Muttil, C. Prego, L. Garcia Contreras, B. Pulliam, J. Fallon, C. Wang, A.J. Hickey and D. A. Edwards Immunization of guinea pigs with novel Hepatitis B antigen as nanoparticle aggregate powders administered by the pulmonary route AAPS journal (2010) 12 (3), 330-337
D. Lu, L. Garcia Contreras, P. Muttil, D. Padilla, D. Xu, J. Liu, M. Braunstein, D. N. McMurray, A. J. Hickey Pulmonary immunization using antigen 85-B polymeric microparticles to boost tuberculosis immunity AAPS journal (2010) 12 (3), 338-347
A. B. Yadav, P. Muttil, A. K. Singh, R. K. Verma, M. Mohan, A. K. Agrawal, A. S. Verma, S. K. Sinha, A. Misra Microparticles induce variable levels of activation in macrophages infected with Mycobacterium tuberculosis Tuberculosis (2010) 90 (3), 188-196
L. Garcia-Contreras, J. C. Sung, P. Muttil, D. Padilla, M. Telko, J. L. VerBerkmoes, K. J. Elbert, A. J. Hickey, and D. A. Edwards Dry powder PA-824 aerosols for the treatment of tuberculosis in guinea pigs Antimicrobial Agents and Chemotherapy (2010) 54 (4), 1436-1442
P. Muttil, C. Wang, A.J. Hickey Inhaled drug delivery for tuberculosis therapy (Expert review) Pharmaceutical research (2009) 26 (11), 2401-2416
A. B. Yadav, R. Sharma, P. Muttil, A. K. Singh, R. K. Verma, M. Mohan, S. K. Patel, A. Misra Inhalable microparticles containing isoniazid and rifabutin target macrophages and "Stimulate the Phagocyte" to achieve high efficacy Indian J Exp Biol (2009) 47 (06), 469-474
C. Wang, P. Muttil, D. Lu, A. Beltran-Torres, L. Garcia-Contreras and A.J. Hickey Screening for potential adjuvants administered by the pulmonary route for tuberculosis vaccines. AAPS journal (2009) 11(1),139-47
L. Garcia-Contreras, Y-L Wong, P. Muttil, D. Padilla, J. Sadoff, J. Derousse, W.A. Germishuizen, S. Goonesekara, K. Elbert, B.R. Bloom, R. Miller, P.B. Fourie, A.J. Hickey and D. Edwards Immunization by a bacterial aerosol. Proceedings of the National Academy of Science USA (2008) 105 (12), 4656-60
J. Kaur, P. Muttil, R. K. Verma, K. Kumar, A. B. Yadav, R. Sharma and A. Misra A hand-held apparatus for "nose-only" exposure of mice to inhalable microparticles as a dry powder inhalation targeting lung and airway macrophages. European Journal of Pharmaceutical Sciences (2008) 34 (1), 56-65
P. Muttil, J. Kaur, K. Kumar, A. B. Yadav, R. Sharma and A. Misra Inhalable microparticles containing large payload of anti-tuberculosis drugs. European Journal of Pharmaceutical Sciences (2007) 32 (2), 140-150
R. Sharma, P. Muttil, A. B. Yadav, S. K. Rath, V. K. Bajpai, U. Mani, and A. Misra Uptake of inhalable microparticles affects defence responses of macrophages infected with Mycobacterium tuberculosis H37Ra. Journal of Antimicrobial Chemotherapy (2007) 59, 499-506
The University of New Mexico’s Doctor of Pharmacy program is accredited by the Accreditation Council for Pharmacy Education, 20 North Clark Street, Suite 2500, Chicago, IL 60602-5109, TEL (312) 664-3575 , FAX (312) 664-4652, URL http://www.acpe-accredit.org/
07/13/2011 05:07:33 PM -0600.