DNA Research Services


DNA and RNA may be synthesized at 0.2 µmole, 1.0 µmole, and 10 µmole scales. The normal method of synthesis is 3' to 5', but oligonucleotide segments can be synthesized in the opposite sense, 5' to 3', for the production of 5'-5' or 3'-3' linkages for antisense studies or structural studies.

Other modifications include:

Phosphorylation of the 5'-terminus Biotin labeling Cholesterol labeling DNP labeling Dancyl labeling Acridine labeling (Intercalating agent) Psoralen labeling (cross linking) Dye labeling: 5'-Fluorescein (6-FAM) 5'-Tetrachloro-Fluorecein (TET) 5'-Hexachloro-Fluorescein (HEX) Cyanine (Cy3 and Cy5) Rhodamine (TAMRA) Duplex Effects
Degeneracy strategies Chain Terminators Structural/Activity Relationships Halogenated nucleosides 8-oxo purine monomers 2'-5' Linked oligos Spacer modifiers 3'-modifiers terminus modifiers Convertible nuleosides Cyclic oligonucleosides Fluorescent nucleosides
and, the anti-viral drug: Cytosine Arabanoside can be incorporated into synthetic oligonucleotides.

 

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