DNA Research Services

DNA Sequencing

An automated instrument system performs electrophoretic separation and spectral detection of dye-labeled DNA fragments, to determine base sequence, fragment size, or relative quantity.

SEQUENCING reactions are performed with fluorescent labels: four different dyes identify the A,C, G, and T extension reactions. Commonly employed polymerases provide primer extension.

The Center requests that you provide the primer for the sequencing reaction. For each reaction requested, please provide 5 µL of primer, at a concentration of  1  pmol/µL.

The template sample should be provided as follows:

SAMPLE REQUIREMENTS:

SAMPLE TYPE		PROVIDE 10 µL/reaction AT THIS CONCENTRATION
Plasmid	-Double-stranded	100 ng/µL
	- Single Stranded	10 ng/µL
PCR product	- 100-199 base pairs	0.5 ng/µL
	- 200-499 bp	1.0 ng/µL
	- 500-999 bp	2.5 ng/µL
	- 1000-2000 bp	5.0 ng/µL
	> 2000 bp	10.0 ng/µL

Order Form