DNA Research Services
DNA Sizing
For DNA sizing, labeling reactions use one fluorescent dye for each sample. The Gene Mapper analysis software uses the automated fluorescence detection capabilities of the 3100 Genetic Analyzer instrument to size and quantify DNA fragments. Running a size standard in the same lane promotes accurate quantitative results. Two size standards are available, depending on the other dyes used: ROX (red) or LIZ (orange).
The ROX size standard can be used with the following fluorescent dye labels: 6-FAM (blue), HEX (green), & NED (yellow).
GeneScan™ 500 ROX® Size Standard is designed for sizing DNA fragments in the 35-500 bp range and providess 16 single-stranded fragments of 35, 50, 75, 100, 139, 150, 160, 200, 250, 300, 340, 350, 400, 450, 490, and 500 bps.
The LIZ size standard can be used with the following fluorescent dye labels: 6-FAM (blue), VIC (green), NED (yellow), & PET (red).
GeneScan™ 500 LIZ™ Size Standard, (designed for use with the AmpFLSTR® Identifiler™ Kit and 5-dye ABI PRISM® Custom Primers).
GeneScan™ 600 LIZ® Size Standard is a new size standard that has been designed for improved fragment sizing and increased throughput enabling accurate and reproducible allele calls in fragment analysis and genotyping assays. The standard, which can be used for sizing in the 20-600 bp range contains 35 fragments that are labeled with the 5th dye LIZ®. The size standard peaks are 20, 40, 60, 80, 100, 114, 120, 140, 160, 180, 200, 214, 220, 240, 260, 280, 300, 314, 320, 340, 360, 380, 400, 414, 420, 440, 460, 480, 500, 514, 520, 540, 560, 580 and 600 bps.
Other size standards are available for different nucleotide ranges: 120 LIZ, 350 ROX, 1000 ROX, and 2500 ROX.