B.S., Microbiology, Soochow University, Taiwan
M.S., Molecular Genetics, Ohio State University
Ph.D., Molecular Genetics, Ohio State University
Post Doctoral Fellow, Howard Hughes Medical Institute, Johns Hopkins University
Research Faculty, Johns Hopkins University School of Medicine

Office: 258 BMSB
Telephone: (505) 272-8816
E-mail: ahu@salud.unm.edu

RESEARCH INTERESTS
Molecular Bases of Human Genetic Disease and Programmed Cell Death in Health and Disease

Student Projects
Bcl-2 Family Members, Apoptosis and Cancer
Molecular Enzymology of Proline Metabolic Enzymes

SELECTED PUBLICATIONS

Books Chapters

X. Steve Fu, C.-A. A. Hu*, J. Chen, J. Wang and K. J. Ray Liu. (2005) Cancer genomics, proteomics and clinical applications. In "Genomics Signal processing and Statistics."

J. Phang, C.-A. A. Hu and D. Valle (2001) Disorders of proline and hydroxyproline metabolism. In "The Metabolic and Molecular Bases of Inherited Disease." C. R. Scriver, A. L. Beaudet, W. S. Sly and D. Valle eds. 8th edition, McGraw-Hill, Inc.

D. P. S. Verma, C.-A. A. Hu, A. J. Delauney, G.-H. Miao and Z. Hong. (1992) Deciphering proline biosynthesis pathways in plants by direct, trans- and co-complementation in bacteria. In "Biosynthesis and Molecular Regulation of Amino Acids in Plants". B. K. Singh, H. E. Flores and J. C. Shannon eds. pp. 128-138. American Society of Plant Physiologists.

Research Papers

Hu CA, Williams DB, Zhaorigetu S, Khalil S, Wan G, Valle D. Functional genomics and SNP analysis of human genes encoding proline metabolic enzymes. Amino Acids. 2008 May 28. [Epub ahead of print]

Wan G, Zhaorigetu S, Liu Z, Kaini R, Jiang Z, Hu CA. Apolipoprotein L1, a novel BH3-only lipid binding protein, induces autophagic cell death. J Biol Chem. 2008 May 26. [Epub ahead of print]

Hu CA, Phang JM, Valle D. Proline metabolism in health and disease. Amino Acids. 2008 May 27. [Epub ahead of print]

Hu CA, Khalil S, Zhaorigetu S, Liu Z, Tyler M, Wan G, Valle D. Human Delta(1)-pyrroline-5-carboxylate synthase: function and regulation. Amino Acids. 2008 Apr 10. [Epub ahead of print]

Liu Z, Wan G, Heaphy C, Bisoffi M, Griffith JK, Hu CA. A novel loss-of-function mutation in TP53 in an endometrial cancer cell line and uterine papillary serous carcinoma model. Mol Cell Biochem. (2007) 297:179-187.

Hu CA, Donald SP, Yu J, Lin WW, Liu Z, Steel G, Obie C, Valle D, Phang JM. Overexpression of proline oxidase induces proline-dependent and mitochondria-mediated apoptosis. Mol Cell Biochem. (2007) 295:85-92.

Liu Y, Borchert GL, Surazynski A, Hu CA, Phang JM. Proline oxidase activates both intrinsic and extrinsic pathways for apoptosis: the role of ROS/superoxides, NFAT and MEK/ERK signaling. Oncogene. (2006) 25:5640-5647.

Fu XS, Hu CA, Chen J, Wang J, Liu KJR. Cancer genomics, proteomics and clinical applications. In “Genomics Signal processing and Statistics.” (2005)

Liu Y, Borchert GL, Donald SP, Surazynski A, Hu CA, Weydert CJ, Oberley LW, Phang JM. MnSOD inhibits proline oxidase-induced apoptosis in colorectal cancer cells. Carcinogenesis. (2005) 26:1335-1342.

Bender HU, Almashanu S, Steel G, Hu CA, Lin WW, Willis A, Pulver A, Valle D. Functional consequences of PRODH missense mutations. Am J Hum Genet. (2005) 76:409-420.

Liu Z, Lu H, Shi H, Du Y, Yu J, Gu S, Chen X, Liu KJ, Hu CA. PUMA overexpression induces reactive oxygen species generation and proteasome-mediated stathmin degradation in colorectal cancer cells. Cancer Res. (2005) 65:1647-1654.

Liu Z, Lu H, Jiang Z, Pastuszyn A, Hu CA. Apolipoprotein L6, a novel proapoptotic Bcl-2 homology 3-only protein, induces mitochondria-mediated apoptosis in cancer cells. Mol Cancer Res. (2005) 3:21-31.

Gu S, Du Y, Chen J, Liu Z, Bradbury EM, Hu CA, Chen X. Large-scale quantitative proteomic study of PUMA-induced apoptosis using two-dimensional liquid chromatography-mass spectrometry coupled with amino acid-coded mass tagging. J Proteome Res. (2004) 3:1191-200.

Gu S, Liu Z, Pan S, Jiang Z, Lu H, Amit O, Bradbury EM, Hu CA, Chen X. Global investigation of p53-induced apoptosis through quantitative proteomic profiling using comparative amino acid-coded tagging. Mol Cell Proteomics. (2004) 3:998-1008.

Phang J, Hu CA, Valle D. Disorders of proline and hydroxyproline metabolism. In "The Metabolic and Molecular Bases of Inherited Disease".  C. R. Scriver, A. L. Beaudet, W. S. Sly and D. Valle eds. 8th edition, McGraw-Hill, Inc. (2001).

Donald SP, Sun XY, Hu CA, Yu J, Mei JM, Valle D, Phang JM. Proline oxidase, encoded by p53-induced gene-6, catalyzes the generation of proline-dependent reactive oxygen species. Cancer Res. (2001) 61:1810-1815.

Baumgartner MR, Hu CA, Almashanu S, Steel G, Obie C, Aral B, Rabier D, Kamoun P, Saudubray JM, Valle D. Hyperammonemia with reduced ornithine, citrulline, arginine and proline: a new inborn error caused by a mutation in the gene encoding delta¹-pyrroline-5-carboxylate synthase. Hum Mol Genet. (2000) 9:2853-2858.

Camacho JA, Obie C, Biery B, Goodman BK, Hu CA, Almashanu S, Steel G, Casey R, Lambert M, Mitchell GA, Valle D. Hyperornithinaemia-hyperammonaemia-homocitrullinuria syndrome is caused by mutations in a gene encoding a mitochondrial ornithine transporter. Nat Genet. (1999) 22:151-158.

Hu CA, Lin WW, Obie C, Valle D. Molecular enzymology of mammalian delta1-pyrroline-5-carboxylate synthase: Alternative splice donor utilization generates isoforms with different sensitivity to ornithine inhibition. J Biol Chem. (1999) 274:6754-6762.

Geraghty MT, Vaughn D, Nicholson AJ, Lin WW, Jimenez-Sanchez G, Obie C, Flynn MP, Valle D, Hu CAMutations in the delta¹-pyrroline 5-carboxylate dehydrogenase gene cause type II hyperprolinemia. Hum Mol Genet. (1998) 7:1411-1415.

Hu CA, Lin WW, Valle D. Cloning, characterization, and expression of cDNAs encoding human delta¹-pyrroline-5-carboxylate dehydrogenase. J Biol Chem. (1996) 271:9795-800.

Kishor PBK, Hong Z, Miao GH, Hu CA, Verma DPS. Overexpression of delta¹-pyrroline-5-carboxylate synthetase increases proline production and confers osmotolerance in transgenic plants. Plant Physiol. (1995) 108:1387-1394.

Delauney AJ, Hu CA, Kishor PB, Verma DP. Cloning of ornithine delta-aminotransferase cDNA from Vigna aconitifolia by trans-complementation in Escherichia coli and regulation of proline biosynthesis. J Biol Chem. (1993) 268:18673-18678.

Verma DPS, Hu CA, Delauney AJ. Genetic manipulation for proline overproduction and the control of osmoregulation in plants. In "Adaptation of Vegetable and Other Food Crops to Temperature and Water Stress". International Symposium on Adaptation of Vegetable and Other Food Crops to Temperature and Water Stress". Asian Vegetable Research and Development Center. (1993).

Verma DPS, Hu CA, Delauney AJ, Miao GH, Hong Z. Deciphering proline biosynthesis pathways in plants by direct, trans- and co-complementation in bacteria.  In "Biosynthesis and Molecular Regulation of Amino Acids in Plants". B. K. Singh, H. E. Flores and J. C. Shannon eds. pp. 128-138. American Society of Plant Physiologists. (1992).

Hu CA, Delauney AJ, Verma DPS. A bifunctional enzyme (delta¹-pyrroline-5-carboxylate synthetase) catalyzes the first two steps in proline biosynthesis in plants. Proc Natl Acad Sci U S A. (1992) 89:9354-9358.

Verma DPS, Hu CA, Zhang M. Root nodule development: origin, function and regulation of nodulin genes. Physiol. Plant. (1992) 85:253-265.

Wong HC, Hu CA, Yeh HL, Su W, Lu HC, Lin CF. Production, Purification, and Characterization of alpha-Galactosidase from Monascus pilosus. Appl Environ Microbiol. (1986) 52:1147-1152.