Associate Professor

Assoc. Professor , University of New Mexico, 2006-present
Asst. Professor , University of New Mexico, 2000-2006
Asst. Professor , University of California, San Diego, 1996-2000
Post Doctoral Fellow, University of California, San Diego, 1991-1995
Post Doctoral Fellow, Scripps Research Institute, 1989-1991
Ph.D. Cell Biology, University of California, Irvine, 1989
B.S. Biology, State University of New York at Albany, 1983

Office: Basic Research Facility, Room 223D
Telephone: 505-272-5593
E-mail: rorlando@salud.unm.edu      

RESEARCH PROJECTS

• Identify the molecular mechanism of fatty acid uptake by adipocytes.
• Characterize the cooperative effects of cell surface heparan sulfate proteoglycans and fatty acid transporters and lipoprotein receptors.
• Investigate the role of natural product inhibitors in Alzheimer Disease pathologies including microglial activation and amyloid plaque formation.
• Investigate the role of inflammatory mediators in adipose hypertrophy and insulin responsiveness.

PUBLICATIONS
 
Heynekamp, J.J., Weber, W.M., Hunsaker, L.A., Gonzales, A.M., Orlando, R.A., Deck, L.M., Vander Jagt, D.L. ”Substituted trans-stilbenes, including analogs of the natural product resveratrol inhibit the TNFainduced activation of transcription factor NF-kB.” J. Med. Chem.  2006, 49:7182-7189.

Wilsie, L.C., Gonzales, A.M., and Orlando, R.A.  Syndecan-1 Mediates Internalization of apoE-VLDL through a low density lipoprotein receptor-related protein (LRP)-independent, non-clathrin-mediated pathway.  Lipids Health Dis., 5(1):23, 2006.

Candia, B.J., Hines, W.C., Heaphy, C.M., Griffith, J.K., and Orlando, R.A.  Protease nexin-1 expression is altered in human breast cancer.  Cancer Cell Int., 6:16, 2006.

Weber, W.M., Hunsaker, L.A., Gonzales, A.M., Heynekamp, J.J., Orlando, R.A., Deck, L.M., and Vander Jagt, D.L.  TPA-induced up-regulation of activator protein-1 can be inhibited or enhanced by analogs of the natural product curcumin.  Biochem. Pharmacol., 72:928-940, 2006.

Atkins, E., Zamora, S., Candia, B.J., Baca, A., and Orlando, R.A.  Development of a Mammalian Suspension Culture for Expression of Active Recombinant Human Urokinase-type Plasminogen Activator.  Cytotechnology, 49:25-37, 2005.Wilsie, L.C., Chanchani, S., Navaratna, D., and Orlando, R.A.  Cell Surface Heparan Sulfate Proteoglycans Contribute to Intracellular Lipid Accumulation in Adipocytes.  Lipids Health Dis., 4(1):2, 2005.

Schmieder, S., Nagai, M., Orlando, R.A., Takeda, T., and Farquhar, M.G.  Podocalyxin Activates RhoA and Induces Actin Reorganization through NHERF1 and Ezrin in MDCK Cells.  J. Amer. Soc. Nephrol., 15(9):2289-2298, 2004.

Orlando, R.A.  The Low Density Lipoprotein Receptor-related Protein Associates with Calnexin, Calreticulin, and Protein Disulfide Isomerase in Receptor-associated Protein Deficient Fibroblasts.  Exp. Cell Res., 294:244-253, 2004.

Saito, A., Kazama, J.J., Iino, N., Cho, K., Sato, N., Yamazaki, H., Oyama, Y., Orlando, R.A., Shimizu, F., Tabata, Y., and Gejyo, F.  Bioengineered Implantation of Megalin Expressing Cells: An Intracorporeal Therapeutic Model for Uremic Toxin Protein Clearance In Renal Failure.  J. Amer. Soc. Nephrol., 14(8):2025-2032, 2003.

Wilsie, L.C., and Orlando, R.A.  The Low Density Lipoprotein Receptor-related Protein Complexes with Cell Surface Heparan Sulfate Proteoglycans to Regulate Proteoglycan-mediate Lipoprotein Catabolism.  J. Biol. Chem., 278:15758-15764, 2003.

Lou, X., McQuistan, T., Orlando, R.A., and Farquhar, M.G.  GAIP, GIPC and Gai3 are Concentrated in Endocytic Compartments of Proximal Tubule Cells:  Putative Role in Regulating Megalin’s Function.  J. Am. Soc. Nephrol, 13(4):918-927, 2002.

Veinbergs, I., Van Uden, E., Mallory, M., Alford, M., McGiffert, C., DeTeresa, R., Orlando, R., Masliah, E.  Role of Apolipoprotein E Receptors in Regulating the Differential in vivo Neurotrophic Effects of Apolipoprotein E. Exp. Neurol., 170:15-26, 2001.

Orlando, R.A., Takeda, T., Zak, B., Schmieder, S. Benoit, V.M., McQuistan, T., Furthmayr, H., and Farquhar, M.G.  The Glomerular Epithelial Cell Anti-Adhesin, Podocalyxin Associates with the Actin Cytoskeleton Through Interactions with Ezrin. J. Am. Soc. Nephrol., 12(8):1589-1598, 2001.

Takeda, T., McQuistan, T., Orlando, R.A., and Farquhar, M.G.  Loss of the Glomerular Foot Process Organization is Associated with Uncoupling of Podocalyxin from the Actin Cytoskeleton.  J. Clin. Invest., 108(2):289-301, 2001.

Czekay, R.P., Orlando, R.A., Kummel, T., and Farquhar, M.G.  Direct Binding of Occupied Urokinase Receptor (uPAR) to LDL Receptor-related Protein (LRP) is Required for Endocytosis of uPAR and Regulation of Cell Surface Urokinase (uPA) Activity.  Mol. Biol. Cell, 12:1467-1479, 2001.

Van Uden, E., Sagara, Y., Van Uden, J., Orlando, R., Mallory, M., Rockenstein, E., and Masliah, E.  A Protective Role of the Low-Density Lipoprotein Receptor-Related Protein Against Amyloid b-Protein Toxicity. J. Biol. Chem., 275:30525-30530, 2000.

Sarti, M., Farquhar, M.G., and Orlando, R.A.  The Receptor-Associated Protein (RAP) Interacts with Several Resident Proteins of  the Endoplasmic Reticulum Including a Glycoprotein Related to Actin.  Exp. Cell Res., 260:199-207, 2000.

Rader, K., Orlando, R.A., Lou, X., and Farquhar, M.G.  Identification and Localization of ANKR44, a Novel RFXANK Family Member, which Interacts with the Cytoplasmic Domain of Megalin. J. Am. Soc. Nephrol., 11:2167-2178, 2000.

Takeda, T., Go, W.Y., Orlando, R.A., and Farquhar, M.G.  Expression of Podocalyxin Inhibits Cell-Cell Adhesion and Modifies Junctional Properties in Madin-Darby Canine Kidney Cells. Mol. Biol. Cell., 11:3219-3232, 2000.

Van Uden, E., Carlson,G., St George-Hyslop, P., Westaway, D., Orlando, R.A., Mallory, M., Rockenstein, E., and Masliah, E.  Aberrant Presenilin-1 Expression Downregulates LDL Receptor-Related Protein (LRP).  Molecular and Cellular Neuroscience, 14(2):129-140, 1999.

Van Uden, E., Veinbergs, I., Mallory, M., Orlando, R.A., and Masliah, E.   A Novel Role for Receptor-Associated Protein in Somatostatin Modulation:  Implications for Alzheimer’s Disease.  Neuroscience., 88(3):687-700, 1999.

Yamazaki, H., Ullrich, R., Exner, M., Saito, A., Orlando, R.A., Kerjaschki, D., and Farquhar, M.G.  All Four Putative Ligand Binding Domains in Megalin Contain Pathogenic Epitopes Capable of Inducing Passive Heymann Nephritis. J. Am. Soc. Nephrol., 9(9):1638-1644, 1998.

Orlando, R.A., Rader, K., Authier, F., Yamazaki, H., Posner, B.I., Bergeron, J.J.M., and Farquhar, M.G.  Megalin is an Endocytic Receptor for Insulin.  J. Am. Soc. Nephrol., 9(10):1759-1766, 1998.

Kerjaschki, D., Exner, M., Ullrich, R., Susani, M., Curtiss, L.K., Witztum, J.L., Farquhar, M.G. and Orlando, R.A.  Pathogenic Antibodies Inhibit the Binding of Apolipoproteins to Megalin/gp330 in Passive Heymann Nephritis.  J. Clin. Invest., 100(9):2303-2309, 1997.

Czekay, R.-P., Orlando, R.A., Woodward, L., Lundstrom, M., and Farquhar, M.G.  Endocytic trafficking of megalin/RAP complexes: Dissociation of the complexes in late endosomes.  Mol. Biol. Cell, 8(3):517-532, 1997.

Orlando, R.A., Exner, M., Czekay, R.-P., Yamazaki, H., Ullrich, R., Saito, A., Kerjaschki, D., and Farquhar, M.G.  Identification of the Second Cluster of Ligand Binding Repeats in Megalin as a Site for Receptor-Ligand Interactions.  Proc. Natl. Acad. Sci., 94 (6):2368-73, 1997.

Knauer, M.F., Orlando, R.A., and Glabe, C.G.  Cell Surface APP751 Forms Complexes with Protease Nexin 2 Ligands and is Internalized via the Low Density Lipoprotein Receptor-Related Protein (LRP).  Brain Res., 740:6-14, 1996.

Saito, A., Yamazaki, H., Rader, K., Nakatani, A., Ullrich, R., Kerjaschki, D., Orlando, R.A., and Farquhar, M.G.  Mapping Rat Megalin: The Second Cluster of Ligand Binding Repeats Contains a 46-amino acid Pathogenic Epitope Involved in the Formation of Immune Deposits in Heymann Nephritis.  Proc. Natl. Acad. Sci., 93:8601-8605, 1996.

Kerjaschki, D., Ullrich, R., Exner, M., Orlando, R.A., and Farquhar, M.G.  Induction of Passive Heymann Nephritis with Antibodies Specific for a Synthetic Peptide Derived from the Receptor-associated Protein.  J. Exp. Med., 183:2007-2015, 1996.

Farquhar, M.G., Saito, A., Kerjaschki, D., and Orlando, R.A.  The Heymann Nephritis Antigenic Complex: Megalin (gp330) and RAP.  J. Am. Soc. Neph., 6:35-47, 1995.

Orlando, R.A., Kerjaschki, D., and Farquhar, M.G.  Megalin (gp330) Possesses an Antigenic Epitope Capable of Inducing Passive Heymann Nephritis Independent of the Nephritogenic Epitope in RAP.  J. Am. Soc. Neph., 6:61-67, 1995.

Czekay, R.-P., Orlando, R.A., Woodward, L., and Farquhar, M.G.  The Expression of Megalin (gp330) and LRP Diverges During F9 Cell Differentiation.  J. Cell Sci., 108:1433-1441, 1995.

Farquhar, M.G., Kerjaschki, D., Lundstrom, M., and Orlando, R.A.  Gp330 and RAP:  The Heymann Nephritis Antigenic Complex.  Ann. N.Y. Acad Sci., 737:96-113, 1994.

Orlando, R.A., and Farquhar, M.G.  Functional Domains of the Receptor Associated Protein (RAP).  Proc. Natl. Acad. Sci., 91:3161-3165, 1994.

Lundstrom, M., Orlando, R.A., Saedi, M.S., Woodward, L., Kurihara, H., and Farquhar, M.G.  Immunocytochemical and Biochemical Characterization of the Heymann Nephritis Antigenic Complex in Rat L2 Yolk Sac Cells.  Am. J. Pathol., 143:1423-1435, 1993.

Orlando, R.A., and Farquhar, M.G.  Identification of a Cell Line that Expresses a Cell Surface and a Soluble Form of the gp330/RAP Heymann Nephritis Antigenic Complex (HNAC).  Proc. Natl. Acad. Sci., 90:4082-4086, 1993.

Kerjaschki, D., Ullrich, R., Diem, K., Pietromonaco, S., Orlando, R.A., and Farquhar, M.G.  Identification of a Pathogenic Epitope Involved in Initiation of Heymann Nephritis.  Proc. Natl. Acad. Sci., 89:11179-11183, 1992.

Orlando, R.A., Kerjaschki, D., Kurihara, H., Biemesderfer, D., and Farquhar, M.G.  Gp330 Associates with a 44 kDa Protein in the Rat Kidney to Form the Heymann Nephritis Antigenic Complex.  Proc. Natl. Acad. Sci., 89:6698-6702, 1992.

Orlando, R.A., and Cheresh, D.A.  Arginine-Glycine-Aspartic Acid Binding Leading to Molecular Stabilization between Integrin avb3 and Its Ligand.  J. Biol. Chem. 266:19543-19550, 1991.

Wayner, E.A., Orlando, R.A., and Cheresh, D.A.  Integrins avb3 and avb5 Contribute to Cell Attachment to Vitronectin but Differentially Distribute on the Cell Surface.  J. Cell Biol. 113:919-929, 1991.

Knauer, D.J., Orlando, R.A., and Rosenblatt, D.E.  The Glioma Cell-Derived Neurite Promoting Activity Protein is Functionally and Immunologically Related to Human Protease Nexin-I.  J. Cell. Physiol. 132:318-324, 1987.