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TaqMan SNP AssaysThe KUGR facility uses commercially-available (TaqMan) real-time PCR assays to detect single nucleotide polymorphisms in patient samples. These assays are suitable for a few or a few hundred SNP measurements in hundreds of samples. Larger projects should consider the use of alternative methodologies, such as Targeted Genotyping Assays. The TaqMan SNP assays are a proven and highly efficient means of measuring SNPs with a high success rate and high call rate, even using relatively poor-quality DNA, such as samples derived from paraffin-embedded samples. For more information, contact the KUGR facility staff. |
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