Stephanie Ruby, Ph.D.
Molecular Genetics and Microbiology
MSC08 4660
1 University of New Mexico
Albuquerque, NM 87131-0001
Office: CRTC 330
Tel: (505) 272-5830
Fax: (505) 272-6029
E-mail: sruby@unm.edu
Keywords: RNA splicing, snRNPs, spliceosome, regulation of gene expression, DNA-damage response, cancer biology, genomics
Research Interests
Regulation of eukaryotic gene expression in response to DNA damaging agents. Role of small ribonucleoproteins (snRNPs) in splice site recognition and splicing of nuclear precursor messenger RNAs. Intranuclear localization and nucleocytoplasmic transport of RNAs.
Information about post-doctoral positions.
Selected Publications
Ruby, S.W., and Szostak, J.W. (1985). Specific Saccharomyces cerevisiae genes are expressed in response to DNA-damaging agents. Mol Cell Biol 5, 75-84.
Ruby, S.W., and Abelson, J. (1988). An early hierarchic role of U1 small nuclear ribonucleoprotein in spliceosome assembly. Science 242, 1028-1035.
Ruby, S.W., and Abelson, J. (1991). Pre-mRNA splicing in yeast. Trends Genet 7, 79-85.
Ruby, S.W., Chang, T.H., and Abelson, J. (1993). Four yeast spliceosomal proteins (PRP5, PRP9, PRP11, and PRP21) interact to promote U2 snRNP binding to pre-mRNA. Genes Dev 7, 1909-1925.
Ruby, S.W. (1997). Dynamics of the U1 small nuclear ribonucleoprotein during yeast spliceosome assembly. J Biol Chem 272, 17333-17341.
Ruby, S.W. (1999). A yeast spliceosome assay. Methods Mol Biol 118, 365-390.
Ruby, S.W. (1999). Yeast pre-mRNA splicing extracts. Methods Mol Biol 118, 323-349.
Awasthi, S., Palmer, R., Castro, M., Mobarak, C.D., and Ruby, S.W. (2001). New roles for the Snp1 and Exo84 proteins in yeast pre-mRNA splicing. J Biol Chem 276, 31004-31015.
Abu Dayyeh, B.K., Quan, T.K., Castro, M., and Ruby, S.W. (2002). Probing interactions between the U2 small nuclear ribonucleoprotein and the DEAD-box protein, Prp5. J Biol Chem 277, 20221-20233.
Tringe, S.G., Wagner, A., and Ruby, S.W. (2004). Enriching for direct regulatory targets in perturbed gene-expression profiles. Genome Biol 5, R29.
Allen, C., Buttner, S., Aragon, A.D., Thomas, J.A., Meirelles, O., Jaetao, J.E., Benn, D., Ruby, S.W., Veenhuis, M., Madeo, F., et al. (2006). Isolation of quiescent and nonquiescent cells from yeast stationary-phase cultures. J Cell Biol 174, 89-100.
Tringe, S.G., Willis, J., Liberatore, K.L., and Ruby, S.W. (2006). The WTM genes in budding yeast amplify expression of the stress-inducible gene RNR3. Genetics 174, 1215-1228.