Acceptable Methods for Euthanasia of Animals

Method Animals under 125 g Rabbits/Rodents over 125 g under 1 kg Rabbits/Rodents over 1 kg under 5 kg Birds Dogs Cats Nonhuman Primates Farm Animals (e.g. swine, ruminants and horses) Reptiles Amphibians3/Fish
CO2 A A4 A4 A UNA UNA UNA A 5
UNA
A A
Barbiturate Overdose/ Euthanasia Solution, Intraveneous (100 mg/kg) A A A A A A A A A A
Barbiturate Overdose/ Euthanasia Solution, Intraperitoneal (100 mg/kg) A A A A UNA A UNA A6
UNA
A A
Anesthesia and Exsanguination A A A A A A A A A A
Anesthesia and Intraveneous KCl (12 meq/kg) A A A A A A A A A A
Anesthesia and Decapitation A A AWJ A UNA UNA UNA UNA A A
Anesthesia and Cervical Dislocation A A UNA A UNA UNA UNA UNA N/A N/A
Decapitation of Awake Animal AWJ1 AWJ AWJ AWJ1 UNA UNA UNA UNA AWJ AWJ
Cervical Dislocation of Awake Animal AWJ2 UNA UNA AWJ1 UNA UNA UNA UNA N/A N/A
Other               Stunning or electrocution followed by exsanguination may be used for on-farm or slaughterhouse euthanasia. Rifle shot may be used for field euthanasia where restraint is not possible.   A- Immersion in MS222 (tricaine) or benzocaine at 2 g/L water