Animal Research Qualifications and Responsibilities
Anesthesia and Analgesia For Laboratory Animals
Surgery
Minor Procedures
Handling and Restraint
Housing, Animal Husbandry and Care
Euthanasia
In order to use animals for research, teaching or testing, the animal user must fulfill all obligations associated with this privilege. These obligations include:
ARF Surgery Facilities
The ARF maintains surgery facilities for non-rodent (large) animals, equipped
with anesthesia machines, monitoring and support equipment. These facilities
must be used for all survival procedures on animals larger than the laboratory
rat. These facilities are also available for non-survival procedures on large
animals.
Survival Surgery Guidelines
Survival Surgery, regardless of species, must be conducted aseptically. Surgical
procedures on non-rodent species, larger than the laboratory rat, must be
conducted in dedicated surgery facilities (ARF surgery facility).
Rodent Surgeries may be conducted in non-dedicated spaces, such as
laboratories and procedure rooms. Aseptic technique, appropriate anesthesia and
analgesia, adequate intra-operative patient monitoring, and provisions for
post-operative care are minimal requirements for conducting survival surgery in
all species. Aseptic surgery principles address the following: sterilization of
instruments, surgeon's prep, surgical site prep, and all surgical procedures
from skin incision to closure. Anesthesia monitoring is imperative and must be
documented in species larger than mice and rats. Review the UNM-HSC guidelines
for aseptic surgery in rodents, if applicable.
Non-Survival (Terminal, Acute) Surgery Guidelines
Non-Survival Surgery is defined as surgery conducted under general anesthesia,
and the patient is not recovered from the anesthesia. Euthanasia is performed at
the end of the experimental surgery procedures,, usually with an overdose of
anesthetic, administered intravenously. Non-survival procedures require the use
of clean instruments, and provisions for adequate monitoring of depth of
anesthesia.
Minor procedures such as injections, oral dosing, observations, weighing, etc. may be conducted in the animal rooms. The ARF provides procedure rooms with inhalant anesthesia, induction chambers, nose cones, waste gas scavenging devices, and recovery units for those procedures wherein anesthesia may be desirable for restraint or immobilization, such as blood collection, biopsies, iv injections, etc.
Handling and Restraint of all animals, but especially rodents is a skill developed from practice and proper training. Animals should be restrained in a gentle, firm, and quick manner in order to minimize the potential for stress and harm to both the restrained and the restrainer. The ARF offers "hands-on" workshops for individuals requiring an introduction to proper restraint methods.
TopAll research animal housing and husbandry is provide by the ARF staff in accord with the Guide for the Care and Use of Laboratory Animals. If an IACUC approved protocol describes non-standard housing conditions, the ARF staff must be informed of the exemption. A Standard Operating Procedure (SOP) must be developed by the researcher, approved by the ARF veterinarian, and posted in the animal room. If the PI assumes responsibility for the husbandry of the animals, a log/record sheet must be posted in the room that records husbandry duties performed by the research staff.
Euthanasia of Laboratory Animals
Euthanasia is the act of inducing a humane death. In laboratory animals,
euthanasia is most commonly the endpoint of any experiment. It is imperative
that researchers conduct euthanasia with respect for the animal's life and with
the understanding that pain, anxiety, and distress must be minimized during the
procedure. The technique employed for euthanasia should ensure rapid
unconsciousness, followed by cessation of cardiac and respiratory functions, and
finally, loss of brain activity. Whatever method is method is chosen,
consideration must be given to reducing the stress and anxiety associated with
the procedure. The American Veterinary Medical Association panel on Humane
Methods of Anesthesia compiled a report in 2000. This report contains specific
guidelines for each species, and acceptable methods of euthanasia. View this
report at:
http://www.aphis.usda.gov/animal_welfare/downloads/reports_out/euthanasia.pdf.
In order to perform euthanasia on animals, the individual must be adequately
trained and competent in the procedure. Euthanasia methods must be detailed in
the IACUC-approved animal use protocol form. Methods used must include measures
to ensure that death has occurred; that there is no chance for the animal to
recover, and regain consciousness. Training is available through the ARF or the
Office of Animal Care and Compliance.
Euthanasia of Mouse and Rat Fetuses and Neonates
Late-term fetuses and neonatal rodents are capable of experiencing pain and
distress. Consideration must be given to additional requirements to perform
humane euthanasia of late-term fetuses and neonates. The NIH Animal Research
Advisory Council has developed
guidelines for the euthanasia of rodent fetuses and neonates. Review these
recommendations for determining acceptable methods of euthanasia for mouse and
rat fetuses and neonates.
Euthanasia of Rodents Using Carbon Dioxide
Carbon Dioxide Asphyxiation is the most common method employed to euthanize
small rodents (e.g., mice and rats). Proper methodology must be employed to
reduce the potential for anxiety, distress and pain associated with high
concentrations of CO2. The NIH Animal Research Advisory Council has
published
Guidelines for Euthanasia of Rodents Using Carbon Dioxide. The ARF has
posted these recommendations as an SOP (Standard Operating Procedure) in the ARF
necropsy suite. If CO2 euthanasia is used in research laboratories,
the PI must request the proper equipment from the ARF, and post the SOP provided
by the ARF.