Jingyue Jia Cassano, PhD

Key Publications

Jia, J., Bissa, B., Brecht, L., Allers, L., Choi, S., Gu, Y., Zbinden, M., Burge, M.,Timmins, G., Hallows, K.,Behrends, C., Deretic, V. (2020). AMPK, a key regulator of metabolism and autophagy, is activated by lysosomal damage via a novel galectin-directed ubiquitin signal transduction system. Molecular Cell 77, 1-19.

Jia, J., Claude-Taupin, A., Gu, Y., Choi, S., Peters, R., Bissa, B., Mudd, M., Allers, L., Pallikkuth, S., Lidke, K., Salemi, M., Phinney, B., Mari, M., Reggiori, F., Deretic, V. (2020). Galectin-3 Coordinates a Cellular System for Lysosomal Repair and Removal. Developmental Cell, 52(1), 69-87.

Gu, Y., Princely Abudu, Y., Kumar, S., Bissa, B., Choi, S.W., Jia, J., Lazarou, M., Eskelinen, E., Johansen, T., and Deretic, V. (2019). Mammalian Atg8 proteins regulate lysosome and autolysosome biogenesis through SNAREs. The EMBO Journal.

Jia, J., Abudu, Y.P., Claude-Taupin, A., Gu, Y., Kumar, S., Choi, S.W., Peters, R., Mudd, M.H., Allers, L., Salemi, M., et al. (2019). Galectins control MTOR and AMPK in response to lysosomal damage to induce autophagy. Autophagy 15, 169–171.

Jia, J., Abudu, Y.P., Claude-Taupin, A., Gu, Y., Kumar, S., Choi, S.W., Peters, R., Mudd, M.H., Allers, L., Salemi, M., et al. (2018). Galectins Control mTOR in Response to Endomembrane Damage. Molecular Cell 70, 120-135.e8.

Kumar, S., Jain, A., Farzam, F., Jia, J., Gu, Y., Choi, S.W., Mudd, M.H., Claude-Taupin, A., Wester, M.J., Lidke, K.A., et al. (2018). Mechanism of Stx17 recruitment to autophagosomes via IRGM and mammalian Atg8 proteins. Journal of Cell Biology 217, 997–1013.

Claude-Taupin, A., Bissa, B., Jia, J., Gu, Y., and Deretic, V. (2018). Role of autophagy in IL-1β export and release from cells. Seminars in Cell and Developmental Biology 83, 36–41.

Claude-Taupin, A., Jia, J., Mudd, M., & Deretic, V. (2017). Autophagy’s secret life: secretion instead of degradation. Essays in biochemistry, 61(6), 637-647.

Kimura, T., Jia, J., Claude-Taupin, A., Kumar, S., Choi, S.W., Gu, Y., Mudd, M., Dupont, N., Jiang, S., Peters, R., et al. (2017). Cellular and molecular mechanism for secretory autophagy. Autophagy 13, 1084–1085.

Kimura, T., Jia, J., Kumar, S., Choi, S.W., Gu, Y., Mudd, M., Dupont, N., Jiang, S., Peters, R., Farzam, F., et al. (2017). Dedicated SNAREs and specialized TRIM cargo receptors mediate secretory autophagy. The EMBO Journal 36, 42–60.

Bai, F., Ho Lim, C., Jia, J., Santostefano, K., Simmons, C., Kasahara, H., Wu, W., Terada, N., and Jin, S. (2015). Directed Differentiation of Embryonic Stem Cells into Cardiomyocytes by Bacterial Injection of Defined Transcription Factors. Scientific Reports 5.

Jia, J., Bai, F., Jin, Y., Santostefano, K.E., Ha, U.-H., Wu, D., Wu, W., Terada, N., and Jin, S. (2015). Efficient Gene Editing in Pluripotent Stem Cells by Bacterial Injection of Transcription Activator-Like Effector Nuclease Proteins. STEM CELLS Translational Medicine 4, 913–926.

Kim, Y.J., Lee, J.H., Lee, Y., Jia, J., Paek, S.H., Kim, H.B., Jin, S., and Ha, U.H. (2014). Nucleoside diphosphate kinase and flagellin from Pseudomonas aeruginosa induce interleukin 1 expression via the Akt/NF-κB signaling pathways. Infection and Immunity 82, 3252–3260.

Jia, J., Jin, Y., Bian, T., Wu, D., Yang, L., Terada, N., Wu, W., and Jin, S. (2014). Bacterial delivery of TALEN proteins for human genome editing. PLoS ONE 9.

Research

Autophagy, a process that plays a general role in maintaining cytoplasmic homeostasis. Autophagy, in its role of quality control, clears cell-damaging sterile irritants or invading pathogens, removes protein aggregates and disposes of dysfunctional or disused organelles. Autophagy, in its metabolic role, plays a key role in maintaining nutrient and energy homeostasis.

Endomembrane damage is a major threat for cellular homeostasis and cell survival. Lysosomes are the organelles cooperating with endocytic, phagocytic or autophagic system to control and safeguard intracellular quality. Lysosomes also play central roles in regulating cellular signaling and metabolism via mTOR or AMPK signaling pathway.

Lysosomes are vulnerable to endogenous or exogenous factors such as aggregates, pathogens, silica crystal, lysosomotropic drugs, inducing lysosomal membrane damage. Lysosomal damage leads to several responses including inactivation of mTOR signaling (Jia J, Mol Cell, 2018), activation of AMPK signaling (Jia J, Mol Cell, 2020), ESCRT membrane repair, autophagy dominated removal or TFEB nuclear translocation for replacement of damaged lysosomes (Jia J, Dev Cell, 2020).

However, how cells repair, removal and replacement of damaged lysosomes is not fully understood, and how these systems are orchestrated to maintain cellular homeostasis is not known.

My research interests involve understanding the role of autophagy in infection, inflammation and metabolism, and how autophagic regulation on lysosomal homeostasis contributes to human disease. My research is performed in close collaboration with Dr. Vojo Deretic.