Useful Links
UNM Shared Flow Cytometry

MSC 09 5025
1 University of New Mexico
Albuquerque, NM 87131

Physical Location:
Cancer Research Facility (CRF; building 229)
Room 217

Phone: (505) 272-0835

Publications

Please use the following acknowledgment on any published data collected at the UNM Shared Flow Cytometry and High Throughput Screening Resource: 
Data was generated in the Flow Cytometry Shared Resource Center supported by the University of New Mexico Health Sciences Center and the University of New Mexico Cancer Center.
 

Platform Characterization

Kuckuck, F.W., Edwards, B.S., Sklar, L.A. High throughput flow cytometry.  Cytometry 44:83-90, 2001.

Ramirez, S., Aiken, C.T., Andrzejewski, B., Sklar. L.A., Edwards, B.S.  High throughput flow cytometry: validation in micro-volume bioassays.  Cytometry Part A 53A:55-65, 2003.

Jackson, W.C., Kuckuck, F., Edwards, B.S., Mammoli, A., Gallegos, C.M., Lopez, G.P., Buranda, T., Sklar, L.A. Mixing small volumes for continuous high throughput flow cytometry: performance of a mixing Y and peristaltic sample delivery.  Cytometry, 47:183-191, 2002.

Jackson, W.C., Bennett, T.A., Edwards, B.S., Prossnitz, E.R., Lopez, G.P., Sklar, L. A. Performance of in-line microfluidic mixers in laminar flow for high throughput flow cytometry. Biotechniques, 32:220-226, 2002.

Young, S.M., Curry, M.S., Ransom, J.T., Ballesteros, J.A., Prossnitz E.R., Sklar L.A., Edwards B.S.  High-throughput microfluidic mixing and multiparametric cell sorting for bioactive compound screening. J. Biomol. Screen 9:103-11, 2004.

Bartsch, J.W., Tran, H.D., Waller, A., Mammoli, A., Buranda, T. Sklar, L.A., Edwards, B.S.  An investigation of liquid carryover and sample residual for a high-throughput flow cytometer sample delivery system. Anal. Chem. 76: 3810-7, 2004.

Naivar, M. A., Parson, J. D., Wilder, M. E., Habbersett, R. C., Edwards, B.S., Sklar, L. A., Graves, S. W., Jett, J. H., Martin, J. C., Nolan, J. P., Trujillo, J. J., Woods, T. A., and Freyer, J. P. Open, Reconfigurable Cytometric Acquisition System:  ORCAS. Cytometry A 71:915-924, 2007

Edwards BS, Zhu J, Chen J, Carter MB, Thal DM, Tesmer JJ, Graves SW and Sklar LA: Cluster cytometry for high-capacity bioanalysis. Cytometry A 81: 419-29, 2012.

HTS Assay Protocols and Reagents

Edwards, B. S., Young, S. M., Oprea, T. I., Bologa, C., Prossnitz, E., and Sklar, L. A. Biomolecular screening of formylpeptide receptor ligands with a sensitive, quantitative, high-throughput flow cytometry platform. Nature Protocols, 1:59-66, 2006.

Edwards, B. S., Ivnitski-Steele, I., Young, S. M., Salas, V. M., and Sklar, L. A. High-throughput Cytotoxicity Screening by Propidium Iodide Staining. Curr Protocols Cytometry, 9.24: 1-9, 2007.

Saunders, M. J., Kim, H., Woods, T. A., Nolan, J. P., Sklar, L. A., Edwards, B.S., and Graves, S. W. Microsphere-based protease assays and screening application for lethal factor and factor Xa. Cytometry A, 69A: 342-352, 2006.

Dennis MK, Bowles HJC, MacKenzie DA, Burchiel SW, Edwards B.S., Sklar LA, Prossnitz ER, Thompson TA: A multifunctional androgen receptor screening (MARS) assay using the high-throughput HyperCyt flow cytometry system. Cytometry A 73:390-9, 2008.

Strouse, JJ, Young, SM, Mitchell, H, Ye, RD, Prossnitz, ER, Sklar, LA, Edwards, BS. A Novel Fluorescent Cross-Reactive Formylpeptide Receptor/Formylpeptide Receptor-Like 1 Hexapeptide Ligand. Cytometry 75A, 264-270, 2009.

Edwards BS, Young SM, Ivnitsky-Steele I, Ye RD, Prossnitz ER, Sklar LA. High-content screening: flow cytometry analysis. Methods Mol Biol 486:151-65, 2009.

Saunders MJ, Edwards BS, Zhu J, Sklar LA, Graves SW. Microsphere-based flow cytometry protease assays for use in protease activity detection and high-throughput screening. Curr Protoc Cytom 13.12.1-17, 2010.

Simons PC, Young SM, Carter MB, Waller A, Zhai D, Reed JC, Edwards BS, Sklar LA: Simultaneous in vitro molecular screening of protein-peptide interactions by flow cytometry, using six Bcl-2 family proteins as examples. Nature protocols, 6:943-952, 2011.

Chen J, Carter MB, Edwards, BS, Cai H, Sklar LA. High throughput flow cytometry based yeast two-hybrid array approach for large-scale analysis of protein-protein interactions.  Cytometry A 81:90-8, 2012.

Surviladze Z, Young SM and Sklar LA: High-throughput flow cytometry bead-based multiplex assay for identification of Rho GTPase inhibitors. Methods Mol Biol 827: 253-70, 2012.

Screening Studies

Young, S.M., Bologa, C., Prossnitz, E.R., Oprea, T.I., Sklar, L.A., Edwards, B.S. High throughput screening with HyperCyt flow cytometry to detect small molecule formylpeptide receptor ligands. J. Biomol. Screen. 10:374-382, 2005.

Edwards, B. S., Bologa, C., Young, S. M., Balakin, K. V., Prossnitz, E., Savchuck, N. P., Sklar, L. A., and Oprea, T. I. Integration of virtual screening with high throughput flow cytometry to identify novel small molecule formylpeptide receptor antagonists. Mol. Pharmacol. 68:1301-1310, 2005.

Bologa, C. G., Revankar, C. M., Young, S. M., Edwards, B.S., Arterburn, J. B., Tkachenko, S. E., Savchuck, N. P., Sklar, L. A., Oprea, T. I., Prossnitz, E. R. Virtual and biomolecular screening converge on a selective agonist for GPR30. Nature Chem. Biol.2: 207-212, 2006

Winter SS, Lovato DM, Khawaja HM, Edwards B.S., Ivnitski-Steele ID, Young SM, Oprea TI, Sklar LA, Larson RS: High Throughput Screening for Daunorubicin-mediated Drug Resistance Identifies Mometasone Furoate as a Novel ABCB1 Reversal Agent. J Biomol Screen 13: 185-193, 2008. 

Ivnitski-Steele I, Sklar LA, Larson RS, Lovato DM, Oprea TI, Edwards B.S.: High throughput flow cytometry to detect selective inhibitors of ABCB1, ABCC1 and ABCG2 transporters. Assay Drug Dev. Tech Assay Drug Dev Technol 6:263-76, 2008.

Young SM, Bologa CM, Fara D, Bryant BjK, Strouse JJ, Arterburn JB, Ye RD, Oprea TI, Prossnitz E, Sklar LA, Edwards B.S.. Duplex High Throughput Flow Cytometry Screen Identifies Two Novel Formylpeptide Receptor Family Probes. Cytometry 75A, 253-263, 2009.

Dennis MK, Burai R, Ramesh C, Petrie WK, Alcon SN, Nayak TK, Bologa CG, Leitao A, Brailoiu E, Deliu E, Dun NJ, Sklar LA, Hathaway HJ, Arterburn JB, Oprea TI, Prossnitz ER. In vivo effects of a GPR30 antagonist. Nat Chem Biol  5:421-427, 2009.

Haynes MK, Strouse JJ, Waller A, Leitao A, Curpan RF, Bologa C, Oprea TI, Prossnitz ER, Edwards BS, Sklar LA, Thompson TA.  Detection of intracellular granularity induction in prostate cancer cell lines by small molecules using the HyperCyt high throughput flow cytometry system.  J Biomol Screen 14:596-609, 2009.

Saunders MJ, Graves SW, Oprea TI, Sklar LA, Edwards BS.  High throughput multiplex protease screening for Botulinum Neurotoxin Type A light-chain protease inhibitors. Assay Drug Dev Technol 8:37-46, 2010.

Surviladze Z, Waller A, Wu Y, Romero E, Edwards BS, Wandinger-Ness A, Sklar LA. Identification of a small GTPase inhibitor using a high-throughput flow cytometry bead-based multiplex assay. J Biomol Screen 15:10-20, 2010.

Buranda T, Wu Y, Perez D, Jett SD, Bondu-Hawkins V, Ye C, Edwards BS, Lopez GP, Sklar LA, Hjelle B.  Recognition of decay accelerating factor and alpha(v)beta(3) by inactivated hantaviruses: Toward the development of high-throughput screening flow cytometry assays. Anal Chem, 402:151-160, 2010.

Curpan RF, Simons PC, Zhai D, Young SM, Carter MB, Bologa CG, Oprea TI, Satterthwait AC, Reed JC, Edwards BS and Sklar LA: High-throughput screen for the chemical inhibitors of antiapoptotic bcl-2 family proteins by multiplex flow cytometry. Assay Drug Dev Technol 9: 465-74, 2011.

Simons PC, Young SM, Carter MB, Waller A, Zhai D, Reed JC, Edwards BS and Sklar LA: Simultaneous in vitro molecular screening of protein-peptide interactions by flow cytometry, using six Bcl-2 family proteins as examples. Nat Protoc 6: 943-52, 2011.

Chen J, Young SM, Allen C, Seeber A, Peli-Gulli MP, Panchaud N, Waller A, Ursu O, Yao T, Golden JE, Strouse JJ, Carter MB, Kang H, Bologa CG, Foutz TD, Edwards BS, Peterson BR, Aube J, Werner-Washburne M, Loewith RJ, De Virgilio C and Sklar LA: Identification of a Small Molecule Yeast TORC1 Inhibitor with a Multiplex Screen Based on Flow Cytometry. ACS Chem Biol 7: 715-22, 2012.

Chan CY, Prudom C, Raines SM, Charkhzarrin S, Melman SD, De Haro LP, Allen C, Lee SA, Sklar LA and Parra KJ: Inhibitors of V-ATPase proton transport reveal uncoupling functions of tether linking cytosolic and membrane domains of V0 subunit a (Vph1p). J Biol Chem 287: 10236-50, 2012.

Holmes AR, Keniya MV, Ivnitski-Steele I, Monk BC, Lamping E, Sklar LA and Cannon RD: The monoamine oxidase A inhibitor clorgyline is a broad-spectrum inhibitor of fungal ABC and MFS transporter efflux pump activities which reverses the azole resistance of Candida albicans and Candida glabrata clinical isolates. Antimicrob Agents Chemother 56: 1508-15, 2012.

Agola JO, Hong L, Surviladze Z, Ursu O, Waller A, Strouse JJ, Simpson DS, Schroeder CE, Oprea TI, Golden JE, Aube J, Buranda T, Sklar LA and Wandinger-Ness A: A Competitive Nucleotide Binding Inhibitor: In Vitro Characterization of Rab7 GTPase Inhibition. ACS Chem Biol: 2012 [Epub ahead of print].

Reviews

Waller, A., Simons, P.C., Biggs, S.M., Edwards, B.S., Prossnitz, E.R., Sklar, L.A. Techniques: GPCR assembly, pharmacology and screening by flow cytometry. Trends Pharmacol Sci. 25:663-9, 2004.

Edwards, B. S., Young, S. M., Saunders, M. J., Bologa, C., Oprea, T. I., Ye, R. D., Prossnitz, E. R., Graves, S. W., and Sklar, L. A. High Throughput Flow Cytometry for Drug Discovery. Expert Opin Drug Discovery, 2: 685-696, 2007.

Sklar LA, Carter MB, Edwards BS.  Flow Cytometry in Drug Discovery, Receptor Pharmacology, and High Throughput Screening. Curr. Opin. Pharmacol. 7:527-534, 2007.

Arterburn JB, Oprea TI, Prossnitz ER, Edwards BS, Sklar LA. Discovery of selective probes and antagonists for G-protein-coupled receptors FPR/FPRL1 and GPR30. Curr Top Med Chem  9:1227-1236, 2009.

Sklar LA, Edwards BS (2010) HTS flow cytometry, small molecule discovery, and the NIH Molecular Libraries Initiative. In: Flow Cytometry in Drug Discovery and Development (Litvin V, and Marder P, eds) pp 71-98, John Wiley & Sons, Hoboken, NJ.

Sklar LA, and Edwards BS. High thoughput flow cytometry for discovery at UNMCMD and the NIH Molecular Libraries Initiative. Drug Discovery World 13:35-46, 2010.