Key Publications

Pu, Jing, Tal Keren-Kaplan, Juan S. Bonifacino. “A Ragulator-BORC Interaction Controls Lysosome Positioning in Response to Amino Acid Availability.” Journal of Cell Biology (2017) 216 (12), 4183-4197

(Preview: Colaço, Alexandria, and Marja Jäättelä, J Cell Biol 216, no. 12 (2017): 3895-3898.)

Jia, Rui, Guardia M. Guardia, Jing Pu, Yu Chen, and Juan S. Bonifacino. “BORC Coordinates Encounter and Fusion of Lysosomes with Autophagosomes.” Autophagy (2017). DOI: 10.1080/15548627.2017.1343768

Guardia, Carlos M., Ginny G. Farías, Rui Jia, Jing Pu, Juan S. Bonifacino. “BORC Functions Upstream of Kinesins 1 and 3 to Coordinate Regional Movement of Lysosomes along Different Microtubule Tracks.” Cell Report 17, no. 8 (2016): 1950-1961.

Pu, Jing, Carlos M. Guardia, Tal Keren-Kaplan, and Juan S. Bonifacino. "Mechanisms and functions of lysosome positioning." Journal of Cell Science (2016): jcs-196287.

Schindler, Christina, Yu Chen, Jing Pu, Xiaoli Guo, and Juan S. Bonifacino. "EARP is a multisubunit tethering complex involved in endocytic recycling." Nature Cell Biology 17, no. 5 (2015): 639-650.

Pu, Jing, Christina Schindler, Rui Jia, Michal Jarnik, Peter Backlund, and Juan S. Bonifacino. "BORC, a Multisubunit Complex that Regulates Lysosome Positioning." Developmental Cell 33, no. 2 (2015): 176-188.

(Preview: Langemeyer, Lars, and Christian Ungermann. Developmental cell 33, no. 2 (2015): 121-122.)

Zhang, Peng, Huimin Na, Zhenglong Liu, Shuyan Zhang, Peng Xue, Yong Chen, Jing Pu, Gong, Peng, Xun Huang, Fuquan Yang, Zhensheng Xie, Tao Xu, Pingyong Xu, Guangshuo Ou, Shaobing O Zhang, Pingsheng Liu. "Proteomic study and marker protein identification of Caenorhabditis elegans lipid droplets." Molecular & Cellular Proteomics 11, no. 8 (2012): 317-328.

Pu, Jing, and Pingsheng Liu. "Fatty acids stimulate glucose uptake by the PI3K/AMPK/Akt and PI3K/ERK1/2 pathways." Protein Phosphorylation in Human Health. Rijeka: InTech10 (2012): 52456.

Ding, Yunfeng, Li Yang, Shuyan Zhang, Yang Wang, Yalan Du, Jing Pu, Gong Peng, Yong Chen, Huina Zhang, Jinhai Yu, Haiying Hang, Peng Wu, Fuquan Yang, Hongyuan Yang, Alexander Steinbüchel, Pingsheng Liu. "Identification of the major functional proteins of prokaryotic lipid droplets." Journal of Lipid Research 53, no. 3 (2012): 399-411.

Zhang, Huina, Yang Wang, Jing Li, Jinhai Yu, Jing Pu, Linghai Li, Hongchao Zhang, Shuyan Zhang, Gong Peng, Fuquan Yang, Pingsheng Liu. "Proteome of skeletal muscle lipid droplet reveals association with mitochondria and apolipoprotein aI." Journal of Proteome Research 10, no. 10 (2011): 4757-4768.

Pu, Jing, Gong Peng, Linghai Li, Huimin Na, Yanbo Liu, and Pingsheng Liu. "Palmitic acid acutely stimulates glucose uptake via activation of Akt and ERK1/2 in skeletal muscle cells." Journal of Lipid Research 52, no. 7 (2011): 1319-1327.

Pu, Jing*, Cheol Woong Ha*, Shuyan Zhang, Jong Pil Jung, Won-Ki Huh, and Pingsheng Liu. "Interactomic study on interaction between lipid droplets and mitochondria." Protein & Cell 2, no. 6 (2011): 487-496.

Peng, Gong, Linghai Li, Yanbo Liu, Jing Pu, Shuyan Zhang, Jinhai Yu, Junjie Zhao, and Pingsheng Liu. "Oleate blocks palmitate-induced abnormal lipid distribution, endoplasmic reticulum expansion and stress, and insulin resistance in skeletal muscle." Endocrinology 152, no. 6 (2011): 2206-2218.

Li, Zhen-Ya, Hui-Min Na, Gong Peng, Jing Pu, and Pingsheng Liu. "Alteration of microRNA expression correlates to fatty acid-mediated insulin resistance in mouse myoblasts." Molecular BioSystems 7, no. 3 (2011): 871-877.

Pu, Jing, Xuelin Zhang, and Pingsheng Liu. "Progress in Studies on the Interaction between Lipid Droplets and Mitochondria." Acta Biophysica Sinica 28, no. 2 (2012)

Zehmer, John K., Youguo Huang, Gong Peng, Jing Pu, Richard GW Anderson, and Pingsheng Liu. "A role for lipid droplets in inter-membrane lipid traffic." Proteomics 9, no. 4 (2009): 914.


The Pu lab’s unique approach to dissecting cellular metabolism involves the study of lysosomes, the subcellular organelles that carry out a variety of functions that are essential for the normal workings of the cell, such as the conversion and trafficking of nutrients, metabolic signaling, and autophagy. Most of these functions rely on lysosome dynamics; namely, the ability of lysosomes to move, their distribution within the cell, and their ability to contact other organelles.

The Pu lab employs cutting edge techniques like large-scale “-omics”, genomic editing, and live cell imaging to elucidate the complex molecular machinery that regulates lysosome dynamics, and examines how these mechanisms influence cellular metabolism as well as human disease.

Metabolic reprogramming of cancerous cells confers adaptation to nutrient poor environments, helping to fulfill the energetic and material demands of cell proliferation. This process has important implications in many different disease states, including cancer and metabolic disorders.

As a member of Autophagy, Inflammation, and Metabolism (AIM) Center, Dr. Pu is interested in using the expertise and resources of the AIM Center to tease apart the molecular pathways that involve errors in human metabolism which can lead to disease states including the development of various cancers.

The Pu lab is currently looking for new members! In addition to her scientific pursuits Dr. Pu is an enthusiastic mentor and has extensive hands-on experience in training upcoming cell and molecular biologists. Prospective graduate students and post-doctoral fellows may contact Dr. Pu at, and are encouraged to attach a cover letter and CV.